Transforming growth factors (TGFs) are biologically active polypeptides that reversibly confer the transformed phenotype on cultured cells. TGF-alpha shows about 40% sequence homology with epidermal growth factor including 6 conserved cysteine residues, which form 3 intramolecular disulfide bonds. TGF-alpha competes with EGF for binding to the EGF receptor, stimulating its phosphorylation and producing a mitogenic response.
Human TGF-alpha or Sacroma growth factor, TGF-type I, ETGF (precursor 160aa, mature form 50aa, aa40-89; chromosome 2p13) Interacts with the PDZ domains of SDCBP and SNTA1 and Contains 1 EGF-like domain. The interaction with SDCBP, is required for the targeting to the cell surface.
TGF-alpha is synthesized and secrted by monocytes, keratinocytes and many normal and tumor cells and tissues.Mice genetically engineered to be lacking TGF-alpha expression showed no abnormalities except for the detection of a "wavy" hair coat.
Source:Recombinant protein corresponding to human TGF-alpha, a 50aa peptide, expressed in E. coli.
Molecular Weight:~5.5kD
Biological Activity:Determined by the dose-dependent stimulation of thymidine uptake by BALB/c 3T3 cells is <0.2 ng/ml.
Specific Activity:~5x10e6units/mg
Endotoxin:<0.1ng/ug (<1EU/ug)
AA Sequence:VVSHFNDCPD SHTQFCFHGT CRFLVQEDKP ACVCHSGYVG ARCEHADLLA
Storage and Stability:Lyophilized powder may be stored at -20°C. Reconstitute with sterile buffer or ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：100ug
Source Antigen：Recombinant, E. coli
Grade：Highly Purified
Purity：~98% (SDS-PAGE, HPLC)
Form：Supplied as a lyophilized powder in PBS. No preservative added. Reconstitute with sterile ddH2O or PBS.
