Celiac disease is an enteropathy that is characterized by intestinal lesions of variable severity. Tissue-type transglutaminase (tTG) is believed to be the predominant autoantigen for celiac disease and the corresponding autoantibodies show higher sensitivity and specificity than anti-gliadin antibodies. Highly pure recombinant human tTG is now available to replace the traditionally used tTG fraction from guinea pig. Tissue-type transglutaminase antigens have been specifically modified for improved handling: exchange of an active site amino acid eliminates the protein cross-linking activity of the enzyme, while maintaining the native three-dimensional structure and the enzyme's secondary GTPase activity. This engineering assures reproducible properties of the antigen preparations through the absence of variable and ill-defined covalent aggregates of tTG antigen and host cell proteins. By point mutation of the active center the catalytic transglutaminase activity has been eliminated, resulting in increased stability during storage and coating.
Source:Recombinant protein corresponding to human Tissue Transglutaminase, fused to a 6x his-tag, expressed in Sf9 insect cells.
Molecular Weight:~78kD
Specificity:Binds IgGA and IgG human TG auto-antibodies.
Applications:Suitable for use in Western Blot with monoclonal anti-hexa-His-tag antibody and standard ELISA test (checker-board analysis of positive/negative sera panels, immuno-dot test). Suitable for biotinylation and iodination. Other applications not tested.
Recommended Dilution:ELISA: 0.6-1.4ug/ml, (depending on the type of ELISA plate and coating buffer)
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：5ug
Source Antigen：Recombinant, sf9 insect cells
Grade：Highly Purified
Purity：~95% (SDS-PAGE)
Form：Supplied as a liquid in 16mM HEPES, pH 8.0, 320mM sodium chloride, 20% glycerol.
