Thyroid-stimulating hormone (also known as TSH or thyrotropin) is a hormone synthesized and secreted by thyrotrope cells in the anterior pituitary gland which regulates the endocrine function of the thyroid gland. TSH stimulates the thyroid gland to secrete the hormones thyroxine (T4) and triiodothyronine (T3). TSH production is controlled by a Thyrotropin Releasing Hormone, (TRH), which is manufactured in the hypothalamus and transported to the Anterior Pituitary gland, where it increases TSH production and release. Somatostatin is also produced by the hypothalamus, and has an opposite effect on the pituitary production of TSH, decreasing or inhibiting its release. The level of Thyroid hormones (T3 and T4) in the blood have an additional effect on the pituitary release of TSH, When the levels of T3 and T4 are low, the production of TSH is increased, and conversely, when levels of T3 and T4 are high, then TSH production is decreased. This effect creates a regulatory negative feedback loop. TSH is a glycoprotein and consists of two subunits, the alpha and the beta subunit. The alpha subunit is identical to that of human chorionic gonadotropin (HCG), luteinising hormone (LH), follicle-stimulating hormone (FSH). The beta subunit is unique to TSH, and therefore determines its function.
Source:Recombinant protein corresponding to human TSH, a heterodimeric glycoprotein comprised of 2 non-covalently linked subunits-an a subunit of 92aa and a b subunit of 118aa, expressed in CHO cells.
Molecular Weight:~28.5kD
Biological Activity:Measured by the amount of cyclic monophosphate (cAMP) produced by a bovine thyroid-derived microsome preparation in response to the TSH and was found to be ~4IU/mg.
Storage and Stability:Lyophilized powder may be stored at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：10ug
Source Antigen：Recombinant, CHO cells
Grade：Highly Purified
Purity：~98% (RP-HPLC, SDS-PAGE)
Form：Supplied as a lyophilized powder in mannitol, sodium phosphate, sodium chloride. Reconstitute with sterile ddH2O >100ug/ml.
