Thyroid Peroxidase (TPO) represents one of the main autoantigenic targets in autoimmune thyroid disease of humans. Its identity with the formerly so-called `microsomal antigen` has been shown several years ago. As an integral membrane glycoprotein it is restricted to the apical plasma membrane of the follicular epithelial cells and comprises two identical subunits of ~100kD molecular weight. The hemoprotein TPO plays a key role in the thyroid hormone biosynthesis by catalysing both the iodination of tyrosyl residues and the coupling of iodotyrosyl residues in thyroglobulin (TG) to form precursors of the thyroid hormones T4 and T3.
Source:Recombinant protein corresponding to human TPO, a glycosylated, polypeptide chain containing 834aa, fused to 6x His-tag, expressed in Sf9 insect cells.
Molecular Weight:~92.87kD
Applications:Suitable for use in ELISA and Western Blot with monoclonal anti-TPO antibody and monoclonal anti-hexa-His-tag antibody. Other applications not tested.
Recommended Dilution:ELISA: 0.15-0.375ug/ml (depending on the type of plate and coating buffer). Suitable for biotinylation and iodination. Optimal dilutions to be determined by the researcher.
Immunological Functions:1. Binds IgG-type human auto-antibodies. 2. Auto-antibodies to TPO recognize conformation-dependent epitopes. 3. Standard ELISA test (checker-board analysis of positive/negative sera panels, including international reference serum NIBSC 66/387).
Storage and Stability:May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：10ug
Source Antigen：Recombinant, sf9 insect cells
Grade：Highly Purified
Purity：~95% (SDS-PAGE)
Form：Supplied as a liquid in 16mM HEPES, pH 7.6, 160mM sodium chloride, 0.08mM potassium iodide, 20% glycerol
