Thyroglobulin (Tg) is a 660kD, dimeric protein produced by and used entirely within the thyroid gland. In earlier literature, Tg was referred to as "colloid". Tg is used by the thyroid gland to produce the thyroid hormones thyroxine (T4) and triiodothyronine (T3). The active form of triiodothyronine, 3, 5, 3' triiodothyronine, is produced both within the thyroid gland and periphery by 5'-deiodinase (which has been referred to as tetraiodothyronine 5' deiodinase.)Thyroglobulin should not be confused with Thyroxine-binding globulin, a carrier protein responsible for carrying the thyroid hormones in the blood. Tg is produced by the thyroid epithelial cells, called thyrocytes, which form spherical follicles. Tg is secreted and stored in the follicular lumen Via a reaction with the enzyme thyroperoxidase, iodine is covalently bound to tyrosine residues in thyroglobulin molecules, forming monoiodotyrosine (MIT) and diiodotyrosine (DIT).
Thyroxine is produced by combining two moieties of DIT. Triiodothyronine is produced by combining one molecule of MIT and one molecule of DIT. Proteases in lysosomes digest iodinated thyroglobulin, releasing T3 and T4. Patients with Hashimoto's thyroiditis or Graves' disease, frequently develop antibodies against Tg. Tg-specific antibodies help in the diagnosis of these diseases, but they also may be present in apparently healthy euthyroid individuals
Thyroglobulin levels in the blood can be used as a tumor marker for certain kinds of thyroid cancer. Thyroglobulin levels in the blood can also be elevated in cases of Graves' disease. Tg has large number of tyrosine residues and other amino acids (Lysine, glutamic and aspartic acid) for coupling.
Source:Thyroglobulin, from porcine Thyroid glands.
Molecular Weight:~660kD; subunit ~330kD
Applications:Suitable for use as a carrier protein for conjugating small peptides, haptens or proteins to increase antigenicity, as an antigen for ELISA and Western Blot and for affinity column preparation to enrich or remove human anti-Tg antibodies from serum or plasma. Other applications not tested.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C. Reconstitute with sterile buffer. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：10mg
Source Antigen：Porcine Thyroid glands
Grade：Highly Purified
Purity：Highly Purified (~95%)
Form：Supplied as a lyophilized powder in 0.02M NaHCO3. No preservative added. Reconstitute with sterile PBS, pH 7.0, 0.15M sodium chloride.
