Thioredoxin reductase (TrxR/NTR), an enzyme belonging to the flavoprotein family of pyridine nucleotide-disulfide oxidoreductases. Thioredoxin reductase (TrxR), a component of the thioredoxin system, including thioredoxin (Trx) and NADPH, catalyzes the transfer of electrons from NADPH to Trx, acts as a reductant of disulfide-containing proteins and participates in the defense system against oxidative stresses.
Source:Recombinant protein corresponding to yeast TrxR, a single, non-glycosylated, polypeptide chain, expressed in E. coli.
Molecular Weight:~36kD
Specific Activity:~5IU/mg
Unit Definition:One unit equals the change in absorbance at 412nm/minute at 25°C using 0.2mM NADPH, 5mM DTNB (pH 7.0).
Storage and Stability:May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：5ug
Source Antigen：Recombinant, E. coli
Grade：Highly Purified
Purity：~98% (RP-HPLC, SDS-PAGE)
Form：Supplied as a liquid in 20mM phosphate buffer, pH 7.4, 0.15M sodium chloride.
