The inhibitors of apoptosis proteins (IAPs) are a widely expressed gene family of apoptotic inhibitors. The central mechanisms of IAP apoptotic suppression appear to be through direct caspases and pro-caspase inhibition. Recently, a new human gene encoding a structurally and unique IAP designated Survivin has been identified. Survivin (human 142aa, ~16.5kD, chromosome 17q25; Mouse TIAP/Survivin 140aa) contains a single baculovirus IAP repeat and lacks a C-terminal RING finger. An isoform of survivin, Beta-form, that has a 23aa insertion in the BIR-domain has been reported. Mouse survivin is also shown to be alternatively spliced into 3 forms: Long 140aa, 121aa (missing the oiled-coil domain), and 40aa form (missing the IAP repeat and coiled domain). Survivin140 and 121aa inhibits caspase-3 activity. It has the property of oncofetal antigens: highly expressed in less-differentiated embryonic cells or rapidly dividing tumor cell but not in fully differentiated adult tissues. Elevated levels of Survivin are found in human fetal lung, liver, heart, kidney, and gastrointestinal tract. In mouse embryonic tissues, Survivin is detected in most tissues. High level of Survivin was found in most common human cancer, including cancers of the lung, colon, pancreas, prostate, and breast. Expression of Survivin also correlated with the presence of both p53 and bcl-2.
Source:Recombinant protein corresponding to full-length human Survivin, fused to His-tag, expressed in E. coli.
Molecular Weight:~21kD
Applications:Suitable for use as Positive Control for Western Blot. It is not suitable for ELISA or other applications where native protein is required. Other applications not tested.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Source Antigen：Recombinant, E. coli
Grade：Highly Purified
Purity：Highly Purified (~95%)
Form：Supplied in SDS-PAGE sample buffer (reduced). Add 5ul of fresh 2x sample buffer/10ul of the solution prior to heating and loading on gels.
