SARS Coronavirus is an enveloped virus containing three outer structural proteins, namely the membrane (M), envelope (E), and spike (S) proteins. Spike (S)-glycoprotein of the virus interacts with a cellular receptor and mediates membrane fusion to allow viral entry into susceptible target cells. Accordingly, S-protein plays an important role in virus infection cycle and is the primary target of neutralizing antibodies.
Source:Recombinant protein corresponding to SARS-CoV Nucleocapsid, containing 422aa residues of the SARS-CoV Nucleocapsid protein and 15 additional aa, fused to his-tag at N-terminal, expressed in E. coli.
Molecular Weight:~47.8kD
AA Sequence:The aa sequence of the recombinant SARS-CoV Nucleocapsid protein is 100% homologous to aa sequence of the native SARS- CoV Nucleocapsid protein.
Applications:Suitable for use in Western Blot. Other applications not tested.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C. Reconstitute with sterile buffer. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：10ug
Source Antigen：Recombinant, E. coli
Grade：Highly Purified
Purity：~95% (SDS-PAGE)
Form：Supplied as a lyophilized powder in 0.05M acetate, pH 4. Reconstitute with 200ul of sterile 0.1M acetate buffer, pH 4 and let the lyophilized pellet dissolve completely.
