85-2067-71　［取扱停止］S-Adenosylmethionine Decarboxylase, Recombinant, Human, His-Tag (MATA2, MATII, SAMS2, MAT-2A, S-adenosylmethionine Synthetase Isoform type-2, AdoMet Synthetase 2, Methionine Adenosyltransferase 2, Methionine Adenosyltransferase II, MAT2A, AMS2) 10ug　209183

特徴

• Adenosylmethionine decarboxylase (EC 1.1.1.50) is an enzyme that catalyzes the conversion of S-adenosyl methionine to S-adenosylmethioninamine. Polyamines such as spermidine and spermine are essential for cellular growth under most conditions, being implicated in a large number of cellular processes including DNA, RNA and protein synthesis. S-adenosylmethionine decarboxylase (AdoMetDC) plays an essential regulatory role in the polyamine biosynthetic pathway by generating the n-propylamine residue required for the synthesis of spermidine and spermine from putrescein. Unlike many amino acid decarboxylases AdoMetDC uses a covalently bound pyruvate residue as a cofactor rather than the more common pyridoxal 5'-phosphate. These proteins can be divided into two main groups which show little sequence similarity either to each other, or to other pyruvoyl-dependent amino acid decarboxylases: class I enzymes found in bacteria and archaea, and class II enzymes found in eukaryotes. In both groups the active enzyme is generated by the post-translational autocatalytic cleavage of a precursor protein. This cleavage generates the pyruvate precursor from an internal serine residue and results in the formation of two non-identical subunits termed alpha and beta which form the active enzyme.
• Source:Recombinant protein corresponding to human AMD1, a non-glycosylated, polypeptide chain containing 334aa, fused to His-tag at N-terminal, expressed in E. coli.
• Molecular Weight:~38kD
• Applications:Suitable for use in ELISA, Western Blot, Inhibition Assays and MS. Other applications not tested.
• Recommended Dilution:Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.