Resistance to insulin characterizes type 2 diabetes, the most common form of diabetes. There is a strong link between type 2 diabetes and obesity, as most patients tend to be obese. Thiozolidinediones (TZDs), a new class of anti-diabetic, enhances target-tissue sensitivity to insulin. A screen of genes down regulated by TZD in adipocyte led to the discovery of a new protein hormone called resistin (for resistance to insulin). Some other protein related to resistin has been called resistin- related molecules (RELM-alpha and beta). Resistin family of proteins was also identified as proteins (FIZZ1-3, for Found in Inflammatory zone) involved in allegory and inflammation.
RELM-alpha/FIZZ1/PMNG1 (Parasite-induced Macrophage Novel Gene 1 (prepeptides: mouse/rat 111aa; ~77% identity) is 63% related to resistin at the C-terminus (38aa). RELM-gamma is a novel member of the resistin-like molecule/found in inflammatory zone (RELM/FIZZ) family in mice and rats. Highest expression in hematopoietic tissues, suggesting a cytokine-like role for RELM-gamma. RELM-gamma-mRNA is detectable in bone marrow, spleen, and lung as well as in peripheral blood granulocytes. RELM-gamma (mouse aa111-117; rat 111aa; Signal peptide aa1-25) is a secreted protein of ~11kD).
Source:Recombinant protein corresponding to 86aa from mature mouse RELM-gamma, fused to his-tag at N-terminal, expressed in E. coli.
Molecular Weight:~11kD, due to the tags
AA Sequence:TLES IVEKKVKELL ANRDDCPSTV TKTFSCTSIT ASGRLASCPS GMTVTGCACG YGCGSWDIRD GNTCHCQCST MDWATARCCQ LA
Applications:Suitable for use in ELISA, Western Blot controls or Standards. Other applications not tested.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C. Reconstitute with sterile buffer. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：25ug
Source Antigen：Recombinant, E. coli
Grade：Highly Purified
Purity：Highly Purified (~95%)
Form：Supplied as a lyophilized powder in 0.05M acetate buffer, pH 4. Reconstitute in 100-200ul of 0.1M acetate buffer, pH 4.
