Resistance to insulin characterizes type 2 diabetes, the most common form of diabetes. There is a strong link between type 2 diabetes and obesity, as most patients tend to be obese. Thiozolidinediones (TZDs), a new class of anti-diabetic, enhances target-tissue sensitivity to insulin. A screen of genes down regulated by TZD in adipocyte led to the discovery of a new protein hormone called resistin (for resistance to insulin). Resistin, specifically produced and secreted by adipocyte, is present at elevated levels in the blood of obese animals, and is down regulated by fasting and anti-diabetic drugs. Antibody to resistin stimulated glucose uptake and improved insulin sensitivity in obese mice. Some other protein related to resistin has been called resistin-related molecules (RELM-alpha and beta). Resistin family of proteins was also identified as proteins (FIZZ1-3, for Found in Inflammatory zone) involved in allegory and inflammation
RELM-beta/FIZZ2 (prepeptides: mouse 105aa; human 11aa ~45-65% identity with FIZZ1 and 2) is highly related to resistin at the C-terminus region. Its expression is most abundant in colon (proximal and distal colon) but not in white adipose tissue. In mouse, RELM- beta is also detected at low levels in cecum and ileum. In min mouse (mouse model for human familial adenomatosus polyposis), RELM-beta expression was markedly increased in tumors immediately adjacent to normal tissue.
Source:Recombinant protein corresponding to two 83aa chains from mouse RELM-beta, expressed in E. coli.
Molecular Weight: ~18kD
Endotoxin:<0.1ng/1ug of protein
Applications:Suitable for use in ELISA or Standards. Other applications not tested.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C. Reconstitute with sterile buffer or ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：25ug
Source Antigen：Recombinant, E. coli
Grade：Highly Purified
Purity：Highly Purified (~97%) This preparation is not sterile.
Form：Supplied as a lyophilized powder in 10mM citrate buffer, pH 3.0. Reconstitute in 100ul PBS or Tris, pH 7.5-8.0 or ddH2O.
