Reg protein was shown to be stimulated during the regeneration of pancreatic islets. Since then, many Reg-related proteins have been identified in humans and other animals. In human, the four REG family genes, i.e., REG 1 alpha, REG 1 beta, REG-related sequence (RS) and HIP/PAP, have so far been isolated. These Reg-related proteins are classified into four subfamilies according to their aa sequences, but they share a similar structure and physiological function. Reg protein is a growth factor for pancreatic beta cells and also suggests thatthe administration of Reg protein could be used as another therapeutic approach for diabetes mellitus. Human REG cDNA encodes a 166aa protein with a 22aa signal peptide. Reg I was found to be expressed mainly in pancreatic beta and acinoductular cells as well as gastric fundic enterochromaffin-like (ECL) cells.
Source:Recombinant protein corresponding to human REG 1 beta, containing 144aa residues of thehuman REG 1 beta and 12 additional aa residues (his-tag), fused to his-tag at N-terminal, expressed in E. coli.
Molecular Weight:~17.8kD
Specificity:The aa sequence of human REG protein has 68% homology to that of rat Reg protein.
Applications:Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：10ug
Source Antigen：Recombinant, E. coli
Grade：Highly Purified
Purity：~95% (SDS-PAGE)
Form：Supplied as a lyophilized powder in 20mM Tris, pH 8.0. No preservative added. Reconstitute with sterile dH20 to a working concentration of ~0.5mg/ml and let the lyophilized pellet dissolve completely. Product is not sterile! Please filter the product by appropriate sterile filterbefore using it in the cell culture.
