Pentraxins are a family of proteins emerging from genes that are conserved in their carboxy-terminal halves a pentraxin domain and are prototypical acute phase proteins with acquired novel amino-terminal domains. Pentraxins, include C reactive protein (CRP) and Serum Amyloid P component (SAP), which serve as indicators of inflammatory reactions as a result of the exposure of liver cells to cytokines, mainly interleukin-6 (IL-6). PTX3 or TSG14 or long pentraxin (human mature chain 364aa, chromosome 3q25; ~43kD) is structurally related but different form classic pentraxins. PTX3 was clones as an IL-1 inducible gene in endothelial cells, and as TNF-inducible gene in fibroblasts called TSG14. The C-terminal half of PTX3 aligns with CRP and SAP, but the N-terminal half is unique. PTX3 is induced by LPS, IL-1 and TNF-alpha, but not IL-6. During an acute phase response induced by LPS, PTX3 is expressed in many organs (heart, skeletal muscle). PTX3 expression may be indicative of its role in cardiovascular and inflammatory pathology.
Source:Recombinant protein corresponding to aa1-381 from mouse PTX3/TSG14, fused to his-tag at C-terminal, expressed in mouse myeloma cell line NSO.
Molecular Weight:~41kD, reducing ~45-50kD due to glycosylation
Biological Activity:Determined by its ability to bind C1q (human C1q at 5ug/ml) in a functional ELISA.
Endotoxin:<1EU/1ug
Storage and Stability:Lyophilized powder may be stored at -20°C. Reconstitute with sterile buffer. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：25ug
Source Antigen：Recombinant, Mouse myeloma cell line NSO
Grade：Purified
Purity：Purified (~90%) This preparation is not necessarily sterile. It can sterile filtered if necessary.
Form：Supplied as a lyophilized powder in 20mM MOPS, 0.3M sodium chloride, 5mM calcium chloride, pH 7.0, 0.1% BSA. Reconstitute in PBS, 0.1% BSA or HSA >5ug/50ul.
