85-2067-50　Protein-L, Recombinant (Agarose) 2ml　209161

特徴

• Protein L (~36kD) is an immunoglobulin-binding protein isolated from the bacteria Peptostreptococcus magnus. Unlike Protein A and Protein G, which bind primarily through Fc regions (i.e., heavy chain) of immunoglobilins, Protein L binds Ig’s through interactions with the light chains. Because no part of the heavy chain is involved in the binding interaction, Protein L binds a wider range of Ig classes than Protein A or G. Protein L binds to representatives of all classes of Ig’s, including IgG, IgM, IgA, IgE and IgD. Single chain variable fragments (ScFv) and Fab fragments also bind to ProteinL. Despite this wide-ranging binding capability with respect to Ig classes, Protein L is not a universal immunoglobilin- binding protein. Binding of ProteinL to immunoglobulins is restricted to those containing kappa light chains (i.e., k chain of the VL domain). In humans and mice, kappa (k) light chains predominate. The remaining immunoglobulins have lambda (l) light chains. Furthermore, Protein L is effective in binding only certain subtypes of kappa light chains. For example, it binds human VkI, VkIII and VkIV subtypes but does not bind the VkII subtype. Binding of mouse immunoglobulins is restricted to those having VkI light chains.
• The main application for immobilized Protein L is purification of monoclonal antibodies from ascites or culture supernatant that are known to have the kappa light chain. Protein L is extremely useful for purification of VLk-containing monoclonal antibodies from culture supernatant because it does not bind bovine, goat or sheep immunoglobilins, which are present in the media serum supplement. Protein L does not interfere with the antigen-binding site of the antibody, making it useful for immunoprecipitation assays, even using IgM. Protein-L can be utilized where Protein-A or G show poor binding.
• Source:Recombinant protein corresponding to P. magnus Protein L.
• Molecular Weight:~36kD
• Binding Capacity:~5-10mg human IgG/ml settled volume of Protein L-agarose.
• Applications:Suitable for use in Immunoprecipitation. Other applications not tested.
• Recommended Dilution:Immunoprecipitation: 20-50ul suspensionOptimal dilutions to be determined by the researcher.
• Specificity:Binds to Ig’s containing light chains of type kappa I, III, and IV in human and kappa I in mouse. May also be specific for certain kappa subgroups in other species. Protein L binds scfv without interfering with antigen binding. Binds weakly to rabbit Ig’s and does not bind Ig’s from bovine, goat or sheep; nor does it bind to lambda light chains.
• Storage and Stability:May be stored at 4°C. For long-term storage, aliquot and store at 4°C. Do not freeze. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.