Protein disulfide isomerases (PDIs) constitute a family of structurally related enzymes which catalyze disulfide bonds formation, reduction, or isomerization of newly synthesized proteins in the lumen of the endoplasmic reticulum (ER). They act also as chaperones, and are, therefore, part of a quality-control system for the correct folding of the proteins in the same subcellular compartment. PDI has been found to have moderate effects (25-fold) on the rate of oxidative folding of proteins in vitro.Recombinant Human Protein Disulfide Isomerase is involved in disulphide-bond formation and isomerization, as well as the reduction of disulphide bonds in proteins. Recombinant PDI has been found to have moderate effects (25-fold) on the rate of oxidative folding of proteins in vitro.
Source:Recombinant protein corresponding to human Protein Disulfide Isomerase, a single, non- glycosylated, polypeptide chain containing 508aa, fused to 6xHis-tag at N-terminal, expressed in E. coli.
Molecular Weight:~57.12kD
Reductase Activity:~1.0x10e-3 delta 650nm/min-2. By measuring the turbidity increase at 650nm due to insulin reduction (Holmgren, A. (1979) J. Biol. Chem. 254, 9627-9632). The activity is expressed as the ratio of the slope of a linear part of the turbidity curve to the lag time (Martnez- Galisteo, E., Padilla, C. A., Garcia-Alfonso, C., Lopez- Barea, J., and Barcena, J. A. (1993) Biochimie (Paris) 75, 803–809).
Isomerase Activity:~0.5umol active RNase A min-1umol PDI-1. According to the re-activation of reduced and denatured RNase A (Lyles, M. M. and Gilbert, H. F. (1991) Biochemistry 30, 613-619).
Storage and Stability:Lyophilized powder may be stored at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：100ug
Source Antigen：Recombinant, E. coli
Grade：Purified
Purity：Chromatographically purified.
Form：Supplied as a lyophilized powder in 50mM potassium phosphate buffer, pH 7.5. Reconstitute with ddH2O >100ug/ml.
