Transthyretin (TTR) is a serum and cerebrospinal fluid carrier of the thyroid hormone thyroxine (T4) and retinol binding protein bound to retinol. This is how transthyretin gained its name, transports thyroxine and retinol. The liver secretes transthyretin into the blood, and the choroid plexus secretes TTR into the cerebrospinal fluid. TTR was originally called prealbumin[1] because it ran faster than albumin on electrophoresis gels. It functions in concert with two other thyroid hormone-binding proteins (Albumin and TBG) in the serum. In cerebrospinal fluid TTR is the primary carrier of T4, as albumin is not present. TTR also acts as a carrier of retinol (vitamin A) through its association with retinol-binding protein (RBP) in the blood and the CSF. Less than 1% of TTR's T4 binding sites are occupied in blood, which is taken advantage of below to prevent TTRs dissociation, misfolding and aggregation which leads to the degeneration of post-mitotic tissue.
TTR is a 55kD homotetramer with a dimer of dimers configuration that is synthesized in the liver, choroid plexus and retinal pigment epithelium for secretion into the eye. Each monomer is a 127- residue polypeptide rich in beta sheet structure. Association of two monomers forms an extended beta sandwich. Further association of two of these dimers produces the homotetrameric structure and creates the two thyroxine binding sites per tetramer. This dimer- dimer interface comprising the two T4 binding sites is the weaker dimer-dimer interface and is the one the comes apart first in the process of tetramer dissociation. TTR is known to be associated with the amyloid diseases[9] senile systemic amyloidosis (SSA),[10] familial amyloid polyneuropathy (FAP),[11][12] and familial amyloid cardiomyopathy (FAC). It is found in serum/plasma at ~0.30 mg/ml. Functions in the transport of thyroxine, vitamin A, and retinol-binding protein. Clinically, prealbumin is a sensitive indicator of impaired liver function; low levels are found in viral hepatitis, cirrhosis, malnutrition, inflammation, and surgical trauma.
Applications:Suitable for use as Positive Control for Western Blot. Not suitable for ELISA or other applications where native protein is required. Other applications not tested.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Source Antigen：Human plasma
Grade：Purified
Purity：Purified
Form：Supplied in SDS-PAGE sample buffer (reduced). Add 5ul of fresh 2x sample buffer/10ul of the solution prior to heating and loading on gels.
