PPK catalyzes the reversible transfer of phosphate between polyphosphate and ATP. The phosphorylation of ADP to ATP by polyphosphate kinase is by a processive mechanism; the phosphorylation occurs without release of the polymer from the enzyme prior to termination of the reaction.
Source:Recombinant protein corresponding to Polyphosphate Kinase, expressed in Propionibacterium shermanii.
Molecular Weight:~83kD (monomer)
Unit Definition:The amount of Polyphosphate Kinase required to convert 1umole ADP to ATP/minute at pH 7.5, using polyphosphate as phosphate donor.
Specific Activity:~1.12U/mg
Required Cofactors:The reaction requires Mg2+
Storage and Stability:Lyophilized powder may be stored at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Form：Supplied as a lyophilized powder in 100mM potassium phosphate, pH 6.8, 25mM sodium polyphosphate. Reconstitute with 1-10ml sterile ddH2O. For long-term storage it is recommended to add a carrier protein (0.1% BSA or HSA).
