Embryonic vascular system undergoes a series of complex, highly regulated series of events involving differentiation, migration and association of primitive endothelial cells. This process is termed vasculogenesis. A further remodeling of the primitive vascular system forms the mature cardiovascular system. This process is known as angiogenesis (sprouting of new capillary vessels from pre- existing vasculature). Angiogenesis accounts for the formation of vasculature into previously avascular organs such as brain and kidney. Angiogenic activity in the adult is required during the normal tissue repair, and for the remodeling of the female reproductive organs (ovulation and placental development). Certain pathological conditions, such as tumor growth and diabetic retinopathy, also require angiogenesis.
Plasminogen prescursor or profibrinolysin or plasma trypsinogen (human 810aa, chromosome 6q26) is synthesized in the kidney. It is present in plasma and many other extracellular fluids. Plasminogen is the zymogen in the circulating blood from which plasmin is formed. Plasminogen is a single-chain glycoprotein with 790aa residues. Activation to the active form, plasmin, by urokinase involves cleavage at the arg-val bond between residues 560 and 561, resulting in the formation of the 2-chain plasmin molecule held together by 2 disulfide linkages. The heavier chain contains about 411 residues and the lighter chain about 233. The main function of plasmin is the digestion of fibrin in blood clots. Plasmin is a proteolytic enzyme with a specificity similar to that of trypsin. Like trypsin, plasmin belongs to the family of serine proteinases, in which the active site catalytic triad, his-57, asp-102, and ser-195 (chymotrypsin numbering), is situated in the light chain. The mature chain represents aa20-810. Processed active peptide domain is aa20-97. Angiostatin mature chain represents aa98-810. This regions contains 5 structurally related kringles domains K1-5 (Kringle 1, aa103-181; Kringle 2, aa184-262; Kringle 3, aa275-352; Kringle 4, aa377-454; Kringle 5, aa481-560). Each Kringle domain contains 6 conserved cysteines. Kringles 1-5 domains share approx 45-50% sequence homology.
Source:Human plasma plasminogen, from human plasma
Activity:~120U/mg protein
Unit Definition:One unit is defined as the amount of enzyme that will hydrolyze 1.0mmol of N- tosyl-Arg ethyl ester in 30 minutes at 37°C, pH 8.0.
Storage and Stability:Lyophilized powder may be stored at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：120U
Source Antigen：Human plasma
Grade：Highly Purified
Purity：Highly Purified (~95%)
Form：Supplied as a lyophilized powder in 20mM Tris-HCl, 2mM EDTA, pH 7.5. Reconstitute with sterile ddH2O.
