Plasminogen activator inhibitor-1 is the principal inhibitor of tissue plasminogen activator (tPA) and urokinase (uPA), the activators of plasminogen and hence fibrinolysis (the physiological breakdown of blood clots). It is a serine protease inhibitor (serpin) protein (SERPINE1). The other PAI, plasminogen activator inhibitor-2 (PAI-2) is secreted by the placenta and only present in significant amounts during pregnancy. In addition, protease nexinacts as an inhibitor of tPA and urokinase. PAI-1, however, is the main inhibitor of the plasminogen activators.
Source:Plasminogen Activator Inhibitor-1, stable mutant 14-1B1, from human. This human form of PAI-1 contains the following four mutations: K154T, Q139L, M354I and N150H. These mutations combine to confer great stability to the otherwise labile molecule essentially locking it into the active conformation.
Molecular Weight:~43kD
Storage and Stability:May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：10ug
Source Antigen：Human
Grade：Highly Purified
Purity：~95% (RP-HPLC, SDS-PAGE).
Form：Supplied as a liquid in 0.05M sodium phosphate, pH 6.6, 0.15M sodium chloride, 1mM EDTA.
