Paraoxonase 1, also called Esterase-A, is involved in the detoxification of organophosphate insecticides such as parathion. Paraoxonase 1 may also confer protection against coronary artery disease by destroying proinflammatory oxidized lipids present in oxidized low-density lipoproteins (LDLs). PON was identified as an enzyme having organophosphates as its substrates. Reports of the geographic differences in population frequencies of paraoxonase activity and genetic analysis led to uncovering the genetic polymorphism. There are 3 known genotypic forms of paraoxonases. They are coded for by the PON set of genes-PON1, PON2 and PON3-located on the long arm of chromosome 7. The differences between them lie in their location and activity. PON1 is synthesized in the liver and transported along with HDL in the plasma. It functions as an antioxidant; it prevents the oxidation of LDL. Its serum concentration is influenced by inflammatory changes and the levels of serum oxidised-LDL. PON2 is a ubiquitously expressed intracellular protein that can protect cells against oxidative damage
PON3 is similar to PON1 in activity but differs from it in substrate specificity. Additionally, it is not regulated by inflammation and levels of oxidised lipids.
Source:Recombinant protein corresponding to human PON-1, fused to his-tag at N-terminal, expressed in E. coli.
Molecular Weight:~43kD
Applications:Suitable for use as Positive Control for ELISA and Western Blot. Other applications not tested.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：10ug
Source Antigen：Recombinant, E. coli
Grade：Highly Purified
Purity：Highly Purified (~95%)
Form：Supplied as a liquid in PBS, pH 7.4, 50% glycerol.
