p53, the most frequent target of genetic alterations in human cancer, is one of the most widely studied tumor suppressor gene. The most important activities of p53 are its cellular growth arrest and induction of programmed cell death (apoptosis). Human p53 is a single polypeptide chain of 393aa. It has three well- defined functional domains: An N-terminal acidic transactivation domain (TAD), a central DNA-binding domain (DBD), and a C-terminal homo-oligomerization domain (OD). All three domains are important for interaction with target genes. A great majority of tumor- associated missense mutations occur within the DBD and the mutant protein fails to regulate p53 target genes.
Posttranslational modifications of p53 (phosphorylation and acetylation) have been implicated in its physiological role. p53 has at least 4 phosphorylation sites at the N-terminus with Ser15 as an important site in response to DNA damage and a target of the ATM and DNA PK proteins. There are three potential sites within the C-terminus of p53 that may affect its DNA-binding ability. p53 also serve as a substrate for various histone acetylases to enhance it transcriptional activity.
Source:Recombinant protein corresponding to human p53, expressed in baculovirus as GST fusion protein (GST-p53).
Molecular Weight:~80kD
Applications:Suitable for use in Kinase Assays, DNA based Binding Assays and as a Positive Control. Other applications not tested.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：10ug
Source Antigen：Recombinant, baculovirus
Grade：Highly Purified
Purity：Highly Purified (~95%)
Form：Supplied as a liquid in 50mM Tris, pH 8.0, 50mM sodium chloride, 0.25M DT, 0.1mM EDTA, 0.1mM PMSF, 10mM glutathione, 25% glycerol
