MMP-3 enzyme is also known as Stromelysin-1 or as Transin-1 which hydrolyzes natural collagen at physiological pH and temperature. It dissolves the intervertebral nucleus pulposus and annulus fibrosus of Herniated Lumbar Intervertebral Disk. MMP-3 hydrolyzes components of the extracellular matrix like proteoglycan, laminin, fibronectin, gelatin and collagen types III, IV and IX. It also activates pro-MMP-9 and pro-MMP-8 and super activates plasmin activated MMP-1. MMP-3 is secreted as a latent proenzyme and is activated by a variety of proteinases, e.g. plasmin, trypsin, chymotrypsin, cathepsin G or human neutrophil elastase. MMP-3 was found to be capable of activating the precursor of IL1-beta.
Source:Recombinant protein corresponding to a single, non-glycosylated, polypeptide chain containing 230aa from human MMP-3, expressed in E. coli.
Molecular Weight:~22kD
Specific Activity:~1250mU/mg protein (H. Nagase, et al; J. Biol. Chem. 269, 20952, 1994).
Unit Definition:One unit is defined as the amount of enzyme that hydrolyzes 1umol of MCA-Arg-Pro-Lys-Pro- Val-Glu-Nva-Trp-Arg-Lys(DNP)-NH2/min. at 37°C, pH 7.0.
Storage and Stability:Lyophilized powder may be stored at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：5ug
Source Antigen：Recombinant, E. coli
Grade：Highly Purified
Purity：~98% (RP-HPLC, SDS-PAGE)
Form：Supplied as a lyophilized powder. No preservative added. Reconstitute with sterile ddH2O >100ug/ml.
