Matrix metalloproteinase 8 (MMP-8), or neutrophil collagenase, degrades interstitial collagens, acting preferentially on collagen type I. Increased full-length MMP-8 protein was associated with infiltration into the skin of neutrophils, which are the major cell type that expresses MMP-8. MMP-8 is synthesized and stored in specific granules in neutrophil leukocytes. MMP-8 activity is therefore regulated by factors such as surface-bound ligands (IgG or complement components) that release it through degranulation. Once released and activated through proteolytic or oxidative mechanisms, MMP-8 plays a major role in the connective tissue turnover that accompanies inflammatory processes.
Source:Recombinant protein corresponding to human MMP-8, a single, non-glycosylated, polypeptide chain, expressed in E. coli.
Molecular Weight:~75kD
Biological Activity:~100units/ml after activation with APMA by solution assay method (10U=100 assays).
Unit Definition:One unit of collagenolytic activity is defined as the cleavage of 1ug of collagen/minute.
Applications:Suitable for use as a standard for analyzing mammalian collagenase activity. Other applications not tested.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：5ug
Source Antigen：Recombinant, E. coli
Grade：Purified
Purity：~90% (SDS-PAGE).
Form：Supplied as a liquid in 0.05M Tris-HCl buffer, pH 7.5, 0.2M sodium chloride, 5mM calcium chloride, 20uM ZnSO4, 0.1% BSA.
