Matrix metalloproteinase-7 (MMP-7) also known as matrilysin and PUMP (EC 3.4.24.23) cleaves a number of substrates including collagen types IV and X, elastin, fibronectin, gelatin, laminin and proteoglycans. MMP-7 is closely related to the stromelysin family members but is encoded by a different gene. MMP-7 is the smallest of all the MMPs consisting of a pro-peptide domain and a catalytic domain. It lacks the hemopexin-like domain common to other members of the MMPs. MMP-7 is secreted as a 28kD proenzyme and can be activated in vitro by organomercurials and trypsin and in vivo by MMP-3 to a 18kD active MMP-7 enzyme. Once activated, MMP-7 can activate pro-MMP-1 and pro-MMP-9 but not pro-MMP- 2. MMP-7 is widely expressed having been reported in elevated levels in cycling endometrium as well as in colorectal cancers and adenomas, hepatocellular carcinomas, rectal carcinomas, and ~50% of gliomas.
Source:Recombinant protein corresponding to human MMP-7, a single, non- glycosylated, polypeptide chain, expressed in E. coli.
Molecular Weight:~19.13kD
Specific Activity:~3100IU/mg
Unit Definition:One unit is defined as the digestion of 1ug Azocoll/min at 37°C.
Applications:Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilution:ELISA: can be used to coat plates at 1ug/ml and detected with antibodies (1:10-50K for neat serum, 0.5-1ug/ml for affinity pure)Western Blot: 1:1K-5K (neat serum), 1-10ug/ml (affinity pure antibody using ECL technique)Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：5ug
Source Antigen：Recombinant, E. coli
Grade：Highly Purified
Purity：~95% (RP-HPLC, SDS-PAGE)
Form：Supplied as a liquid in 10mM HEPES, pH 7.4, 5mM calcium chloride, 150mM sodium chloride.
