Recombinant Human PEX is a noncatalytic Metalloproteinase Fragment with Integrin Binding Activity and can inhibit cell associated collagenolytic activity both in vitro and in vivo. Moreover, PEX can block angiogenesis and tumor growth in vivo, providing a potentially novel therapeutic approach for diseases associated with neovascularization. The appearance of PEX at sites of neovascularization may not only control normal angiogenesis, but when administered in sufficient quantities, may provide a naturally-occurring therapeutic inhibitor of diseasesassociated with angiogenesis. PEX is expressed as inclusion bodies in E. coli and subsequently refolded in vitro to get biological activity.
Source:Recombinant protein corresponding to aa445-635 from human PEX, expressed in E. coli.
Molecular Weight:~28.45kD
Biological Activity:Measured by HMEC cell line, PEX can inhibit the transmembrane activity of HMEC under the stimulation of VEGF.
Storage and Stability:Lyophilized powder may be stored at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：10ug
Source Antigen：Recombinant, E. coli
Grade：Highly Purified
Purity：Highly Purified (~95%)
Form：Supplied as a lyophilized powder in 2mM Tris, pH 7.4. No preservative added. Reconstitute with sterile ddH2O >100ug/ml.
