Protein melan-A also known as melanoma antigen recognized by T-cells 1 or MART-1 is a protein that in humans is encoded by the MLANA gene. A fragment of the protein, usually consisting of the nine amino acids 27-35, is bound by MHC class I complexes which present it to T cells of the immune system. These complexes can be found on the surface of melanoma cells. Decameric peptides (26-35) are being investigated as cancer vaccines. MART-1 or MELAN-A is recognized by autologous T lymphocytes. Six other melanoma associated antigens recognized by autologous cytotoxic T cells include MAGE-1, Tyrosinase, gp100, gp75, BAGE-1 and GAGE-1. Subcellular fractionation shows that MART-1 is present in melanosomes and endoplasmic reticulum. Antibodies against the MART-1 are used in the medical specialty of anatomic pathology in order to recognize cells of melanocytic differentiation, useful for the diagnosis of a melanoma. The same name is also used to refer to the gene which codes for the antigen. The MART-1/Melan-A antigen is specific for the melanocyte lineage, found in normal skin, the retina, and melanocytes, but not in other normal tissues. It is thus useful as a marker for melanocytic tumors (melanomas) with the caveat that it is normally found in benign nevi as well. Mart1 is involved in melanosome biogenesis by ensuring the stability of GPR143. Plays a vital role in the expression, stability, trafficking, and processing of melanocyte protein PMEL, which is critical to the formation of stage II melanosomes
Source:Recombinant protein corresponding to full-length human MART-1, a putative transmembrane protein consisting of 118aa (mouse 113aa), expressed in E. coli.
Applications:Suitable for use in ELISA, Western Blot and Inhibition Assays. Other applications not tested.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：2ug
Source Antigen：Recombinant, E. coli
Grade：Highly Purified
Purity：Highly Purified (~95%)
Form：Supplied as a liquid in 50mM Tris-Acetate, pH 7.5, 1mM EDTA, 20% glycerol.
