Macrophage Inflammatory Proteins (MIP) belongs to the family of chemotactic Cytokines known as chemokines. In humans, there are two major forms, MIP-1a and MIP-1b that are now officially named CCL3 and CCL4 respectively. Both are major factors produced by macrophages after they are stimulated with bacterial endotoxins. They activate human granulocytes (neutrophils, eosinophils and basophils) which can lead to acute neutrophilic inflammation. They also induce the synthesis and release of other pro-inflammatory cytokines such as interleukin 1(IL-1), IL-6 and TNF-alpha from fibroblasts and macrophages. The genes for CCL3 and CCL4 are both located on human chromosome 17.
Source:Recombinant protein corresponding to human MIP-1a, a single, non-glycosylated, polypeptide chain containing 68aa, fused to his-tag at N-terminal, expressed in E. coli.
Molecular Weight:~7.8kD
Storage and Stability:May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
