Chemokine (C-C motif) ligand 19 (CCL19) is a small cytokine belonging to the CC chemokine B family that is also known as EBI1 ligand chemokine (ELC) and macrophage inflammatory protein-3-beta (MIP-3-beta). CCL19 is expressed abundantly in thymus and lymph nodes, with moderate levels in trachea and colon and low levels in stomach, small intestine, lung, kidney and spleen. The gene for CCL19 is located on human chromosome 9. This chemokine elicits its effects on its target cells by binding to the chemokine receptor chemokine receptor CCR7. It attracts certain cells of the immune system, including dendritic cells and antigen-engaged B cells.
Source:Recombinant protein corresponding to a single, non-glycosylated, polypeptide chain containing 83aa from mouse MIP-3-beta, expressed in E. coli.
Molecular Weight:~9.2kD
Biological Activity:Calculated by the ability to chemoattract human mature dendritic cells using a concentration of 10-100ng/ml.
Protein Content:Protein quantitation was carried out by two independent methods:1. UV spectroscopy at 280nm using the absorbency value of 0.91 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).2. Analysis by RP-HPLC, using a calibrated solution of MIP-3b as a Reference Standard.
Storage and Stability:Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：5ug
Source Antigen：Recombinant, E. coli
Grade：Highly Purified
Purity：~95% (RP-HPLC, SDS-PAGE).
Form：Supplied as a lyophilized powder. No preservative added. Reconstitute with sterile ddH2O >100ug/ml. For long term storage add a carrier protein (0.1% HSA or BSA).
