Lysostaphin, an endopeptidase specific for the cell wall peptidoglycan of staphylococci, is an extremely potent anti-staphylococcal agent. Lysostaphin is used as a research and diagnostic tool. Because it lyses staphylococci efficiently, it is widely used when preparing staphylococcal DNA or other cellular components for genetic and biochemical studies and for the preparation of protoplasts for transformation. Preparation and analysis of bacterial DNA has become a powerful tool used by clinical and other microbiologists in epidemiological studies aimed at tracing sources of infection or bacterial contamination.
Source:Recombinant protein corresponding to Lysostaphin, expressed in Bacillus sphaericus.
Biological Activity:Determined by the decrease in turbidity of a suspension of heat-killed Staphylococcus aureus at pH 8.0, 30°C.
Specific Activity:~4000U/mg
Protein Content:Protein quantitation was carried out by two independent methods:1.UV spectroscopy at 280nm using the absorbency value of 2.02 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis RP-HPLC, using a calibrated solution of Lysostaphin as a Reference Standard.
Storage and Stability:Lyophilized and reconstituted products are stable for 6 months after receipt at -20°C. Reconstitute with 20mM sodium acetate. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：1mg
Source Antigen：Recombinant, Bacillus sphaericus
Grade：Purified
Purity：~93% (RP-HPLC, SDS-PAGE)
Form：Supplied as a lyophilized powder from 20mM sodium acetate, pH 4.2. Reconstitute with 20mM sodium acetate, pH 4.2.
