Antibodies to small peptide/haptens are generally raised by coupling to a large carrier proteins such as KLH (Keyhole Limpet Hemocyanin). KLH mol wt ranges 3-7.5 million. It is used for carrier protein as it offers plenty of free amino, carboxyl and cysteines for coupling various peptides or haptens.
Antibodies are produced to both KLH and the peptide/hapten. Anti-KLH may give non-specific signals in various immunoassays. Anti-KLH antibodies can be removed by solid phase immunoaffinity column chromatography over the KLH-Agarose affinity column or by adsorbing with KLH protein.
Source:KLH, isolated from the hemolymphs of the molllusk Megathura crenulata
Storage and Stability:May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：100mg
Source Antigen：Megathura crenulata
Grade：Purified
Purity：Purified
Form：Supplied as a liquid in 10mM MgSO4., pH 6.5, 5mM BES, 0.9% sodium chloride.
