Interleukin-8 (IL-8) is a chemokine produced by macrophages and other cell types such as epithelial cells. It is also synthesized by endothelial cells, which store IL-8 in their storage vesicles, the Weibel-Palade bodies. When first encountering an antigen, the primary cells to encounter it are the macrophages who phagocytose the particle. Upon processing, they release chemokines to signal other immune cells to come in to the site of inflammation. IL-8 is one such chemokine. It serves as a chemical signal that attracts neutrophils at the site of inflammation, and therefore is also known as Neutrophil Chemotactic Factor.
Source:Recombinant protein corresponding to a single, non-glycosylated polypeptide chain containing 77aa from human Interleukin-8, expressed in E. coli.
Molecular Weight:~8.9kD
Biological Activity:Specific activity of IL8 in chemotaxis of donor PBL neutrophils, threshold concentration corresponding to 25-150ng/ml.
Storage and Stability:Lyophilized powder may be stored at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：5ug
Source Antigen：Recombinant, E. coli
Grade：Highly Purified
Purity：~98% (RP-HPLC, SDS-PAGE)
Form：Supplied as a lyophilized powder. No preservative added. Reconstitute with 100ul sterile ddH2O for ~1mg/ml stock. For long term storage add a carrier protein (0.1% HSA or BSA).
