Interleukin-8 (IL-8) is a chemokineproduced by macrophagesand other cell types such as epithelial cells. It is also synthesized by endothelial cells, which store IL-8 in their storage vesicles, the Weibel-Palade bodies. When first encountering an antigen, the primary cells to encounter it are the macrophageswho phagocytosethe particle. Upon processing, they release chemokinesto signal other immune cells to come in to the site of inflammation. IL-8 is one such chemokine. It serves as a chemical signal that attracts neutrophilsat the site of inflammation, and therefore is also known as Neutrophil Chemotactic Factor. Specific Activity of IL8 in chemotaxis of donor PBL neutrophils, threshold concentration corresponding to 10-100ng/ml.
Source:Recombinant protein corresponding to a single non-glycosylated polypeptide chain, containing 72aa from human Interleukin-8, expressed in E. coli.
Molecular Weight:~8.5kD
Storage and Stability:Lyophilized powder may be stored at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：5ug
Source Antigen：Recombinant, E. coli
Grade：Highly Purified
Purity：~98% (RP-HPLC, SDS-PAGE).
Form：Supplied as a lyophilized powder. No preservative added. Reconstitute in 100ul ddH2O. For long term storage add a carrier protein (0.1% HSA or BSA).
