Influenzavirus B is a genus in the virus family Orthomyxoviridae. The only species in this genus is called "Influenza B virus". Influenza B virus only infects humans and seals. This limited host range is apparently in contrast with those caused by the similar Influenza virus A as both mutate by both genetic drift and reassortment. Influenza-B virus evolves slower than A viruses and faster than C viruses. Influenza-B virus mutates at a rate 2-3 times lower than type A. However, influenza B mutates enough that lasting immunity is not possible. The Influenza B virus capsidis enveloped while its virion consists of a matrix protein + envelope + nucleoprotein complex + nucleocapsid, and a polymerase complex. Influenza B is sometimes spherical and sometimes filamentous. Its 500 or so surface projections are made of hemagglutinin and neuraminidase. The Influenza B virus is 14648 nucleotides long and consists of eight segments of linear negative- sense, single-stranded RNA. The multipartite genome is encapsidated, each segment in a separate nucleocapsid, and the nucleocapsids are surrounded by one envelope.
Source:Influenza B virus, strain Florida 04/06, from Allantoic fluid of 10 days old embryonated eggs, inoculated with influenza B virus, strain Florida 04/06.
Applications:Suitable for use in ELISA. Other applications not tested.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Inactivation:Thimerosal and beta propiolactone treatment.
Storage and Stability:May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size:10ug
Source Antigen:Allantoic fluid of 10 days old embryonated eggs
Grade:Purified
Purity:~90% (Ultracentrifugation with 10-40% sucrose gradient and inactivated)
Form:Supplied as a sterile filtered liquid in 0.1M sodium chloride, 10mM Tris Hcl, 1mM EDTA, pH 8, 0.005 % thimerosal, 0.09% sodium azide.