HtrA2, also called Omi, is a mammalian serine protease at high temperatures and has a chaperone activity at low temperature. The full-length HtrA2 is synthesized as a precursor protein and then targeted to the mitochondria where it is matured by the removal of N-terminal 133 residues. Mature HtrA2 consists of a putative transmembrane domain; an inhibitor of apoptosis protein (IAP)-binding motif; a single C-terminal PDZ domain that mediates protein-protein interactions. Recently, HtrA2 has known to contribute both to caspase-dependent and caspase-independent cell death.
Source:Recombinant protein corresponding to aa134-458 from human HTRA2, fused to his-tag, expressed in E. coli.
Molecular Weight:~32kD
Storage and Stability:May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：10ug
Source Antigen：Recombinant, E. coli
Grade：Purified
Purity：~90% (RP-HPLC, SDS-PAGE)
Form：Supplied as a liquid in 20mM Tris-HCl, pH 8.0, 50mM sodium chloride, 1mM DTT, 20% glycerol.
