85-2066-13　［取扱停止］HSP90B1, Recombinant, Human (GRP94, Hsp90 beta 1, Heat Shock Protein 90 B1, TRA1, GP96, Endoplasmin, Heat Shock Protein 90kD beta Member 1, 94kD Glucose-regulated protein, GP96 homolog, Tumor Rejection Antigen 1) 5ug　209013

特徴

• HSP90B1 is an abundant molecular chaperone resident endoplasmic reticulum (ER) lumenal stress protein which is part of the Hsp90 family. HSP90B1 is involved in maintaining protein homeostasis in the secretory pathway as well as functioning in the intracellular trafficking of peptides from the extracellular space to the MHC class I antigen processing pathway of antigen presentation cells. HSP90B1 has key roles in signal transduction, protein folding, protein degradation, and morphologic evolution. HSP90B1 protein associates with numerous cochaperones and involved in folding of newly synthesized proteins or stabilizing and refolding denatured proteins after stress. HSP90B1 is highly expressed in human gastric carcinoma BGC-823 cells during the whole cell cycle.
• Source:Recombinant protein corresponding to aa22-819 from human HSP90B1, fused to His-tag at N-terminal, expressed in E. coli.
• Molecular Weight:~95kD
• AA Sequence:MRGSHHHHHH VDVDGTVEED QIRELREKSE ISNASDALDK LHVTDTGVGM GQSTSELIGQ SDSNEFSVIA KNLVKKYSQF ESDDEAAVEE RPSKEVEEDE GMASMTGGQQ LGKSREGSRT KFAFQAEVNR IRLISLTDEN TREELVKNLG FGVGFYSAFL DPRGNTLGRG INFPIYVWSS EEEEKKPKTK YKAFYKSFSK MGRDLYDDDD DDEVVQREEE MMKLIINSLY ALSGNEELTV TIAKSGTSEF VADKVIVTSK TTITLVLKEE KTETVEEPME KVEKTVWDWE ESDDPMAYIH KDRWGSMDDE AIQLDGLNAS KNKEIFLREL KIKCDKEKNL LNKMTEAQED HNNDTQHIWE ASDYLELDTI EEEAAKEEKE LMNDIKPIWQ FTAEGEVTFK KSDYIKLYVR NVSRETLQQH WKEFGTNIKL DQYVERMKEK VIYLTEPVDE KESREAVEKE CALVASQYGW FEINPRHPLI SGYLLPDTKA ETAEDTTEDTSILFVPTSAP MMPKYLNFVK VRKTLDMIKK RLAKLLRFQS SRKEAESSPF GKRFQNVAKE KALKDKIEKA QAYQTGKDIS EDDKTVLDLA LSLNIDPDAK GTDEEEETAK RGLFDEYGSK GVVDSDDLPL IADDKYNDTF SHHPTDITSL VERLLKKGYE GVKFDESEKT VVSQRLTESP TNYYASQKKT VVLFETATLR VEEEPEEEPE ESTAEKDEL. RVFITDDFHD KLLKVIRKKL GVIEDHSNRT QDKIYFMAGS YCIQALPEFD FEPLLNWMKD SGNMERIMKA RDMLRRIKED YGDRIERMLR EQDEDEEMDV
• Storage and Stability:May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.