The enzyme horseradish peroxidase, found in horseradish, is used extensively in molecular biology and in antibody amplification and detection, among other things. For example, "In recent years the technique of marking neurons with the enzyme horseradish peroxidase (HRP) has become a major tool. In its brief history, this method has probably been used by more neurobiologists than have used the Golgi stain since its discovery in 1870." Horseradish peroxidase is also highly used in techniques such as Western blotting and ELISAs. HRP is widely used as an enzymatic label in immunoassays. Usually, the enzyme is coupled to antibodies, lectins or haptens. Coupling to antibodies etc. may be performed through the carbohydrate side chains of the HRP.
Source:Horseradish Peroxidase from root extracts of horseradish HRP which consists of the basic isoenzyme.
Molecular Weight:~44kD
Activity:≥250units/mg protein. (25°C, guaiacol as the hydrogen donor, pH 7 and H2O2 as substrate).
RZ:~3.5
Storage and Stability:Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. This product is sensitive to light.
仕様
Size：20mg
Source Antigen：root extracts of horseradish
Grade：Affinity Purified
Purity：Purified by affinity chromatography.
Form：Supplied as a lyophilized powder. No preservative added. Reconstitute with sterile ddH2O not less than 100ug/ml or more than 10mg/ml solutions.
