Integrase is an enzyme produced by the HIV which enables its genetic material to be integrated into the DNA of the infected cell and is a key component in the pre-integration complex. HIV integrase contains 3 domains, an N-terminal HH-CC zinc finger domain which is partially responsible for multimerization, a central catalytic domain and a C-terminal domain. Both Central catalytic domain and C-terminal domains have been shown to bind both viral and cellular DNA. No crystal structure data exists with Integrase bound to its DNA substrates. HIV-1 integrase functions as a dimer or a tetramer. Additionally, several host cellular proteins interact with integrase and may facilitate the integration process.
Source:Recombinant protein corresponding to a non-glycosylated polypeptide chain, containing the HIV-1 immunodominant regions from the p31 protein (intergrase), aa9-289, with a GST-tag at N-terminal, expressed in E. coli.
Specificity:Immunoreactive with all sera of HIV-1 infected individuals.
Applications:Suitable for use as an antigen in ELISA and Western Blot, excellent for early detection of HIV seroconvertors with minimal specificity problems. Other applications not tested.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：2ug
Source Antigen：Recombinant, E. coli
Grade：Highly Purified
Purity：Highly Purified (~95%)
Form：Supplied as a liquid in 25mM Tris-HCl, pH 8.0, 0.2% Triton-X, 1.5M urea, 50% glycerol.
