Recombinant DNA technology allows the addition of short pieces of well-defined tags, “peptides” or proteins at the amino or c-terminus of target genes, which can provide 'affinity handles' designed to bind specific matrices. Therefore, tags enables a selective identification and purification of the protein of interest. Poly-His (His x 6) containing proteins can be purified by immobilized metal ion affinity chromatography (IMAC), making use of its high affinity for transition metal ions. This purification system eliminates the harsh conditions required to elute protein from ligand affinity columns. Numerous recombinant fusion proteins have been engineered with histidine tags to allow detection, isolation and purification of fusion proteins. Anti-poly may be used to identify the expression of a poly-histidine fusion protein in bacteria, bacterial lysates or cells and tissues transfected with a poly-histidine fusion protein expression vectors.
Recombinant protein corresponding to poly Hisx6-tag (His-tag), expressed as N-terminal fusion protein in E. coli.
Molecular Weight:~130kD
Applications:Suitable for use as Positive Control for Western Blot. Not suitable for use in ELISA. Other applications not tested.
Recommended Dilutions:Western Blot: load 10 ul/lane. The protein migrates at ~130kD band. Occasionally, other bands may be seen if too much protein is loaded on the gels or too much primary antibodies. Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：100ul
Source Antigen：Recombinant, E. coli
Grade：Highly Purified
Purity：≥95%
Form：Supplied as a liquid in SDS-PAGE sample buffer (reduced).
