Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. The protein encoded by this gene is a cytoplasmic enzyme which belongs to the class II family of aminoacyl-tRNA synthetases. The enzyme is responsible for the synthesis of histidyl-transfer RNA, which is essential for the incorporation of histidine into proteins. The gene is located in a head-to-head orientation with HARSL on chromosome five, where the homologous genes share a bidirectional promoter. The gene product is a frequent target of autoantibodies in the human autoimmune disease polymyositis/dermatomyositis.
Source:Recombinant protein corresponding to human Histidyl-tRNA Synthetase, a single, non- glycosylated, polypeptide chain, expressed in E. coli.
Molecular Weight:~55kD
Protein Content:Protein quantitation was carried out by using 0.25-2.0mg/ml Bradford assay vs. BSA.
Applications:Suitable for use in Western Blot. Other applications not tested.
Recommended Dilution:Western Blot: strongly reactive with human anti Histidyl-tRNA Synthetase antiseraOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：10ug
Source Antigen：Recombinant, E. coli
Grade：Purified
Purity：~90% (RP-HPLC, SDS-PAGE)
Form：Supplied as a liquid in 150mM sodium chloride, pH 7.2, 10mM sodium phosphate, 0.09% sodium azide.
