HCV is a small 50nm, enveloped, single-stranded, positive sense RNAvirus in the family Flaviviridae. HCV has a high rate of replication with ~one trillion particles produced each day in an infected individual. Due to lack of proofreading by the HCV RNA polymerase, the HCV has an exceptionally high mutation rate, a factor that may help it elude the host's immune response. Hepatitis C virus is classified into six genotypes (1-6) with several subtypes within each genotype. The preponderance and distribution of HCV genotypes varies globally. Genotype is clinically important in determining potential response to interferon-based therapy and the required duration of such therapy. Genotypes 1 and 4 are less responsive to interferon-based treatment than are the other genotypes (2, 3, 5 and 6).
Source:Recombinant protein corresponding to the HCV NS4 immunodominant regions, aa1658-1863, fused with b-galactosidase (~114kD) at N-terminus, expressed in E. coli.
Molecular Weight:~19kD
Specificity:Immunoreactive with sera of HCV-infected individuals.
Applications:Suitable for use as an antigen in ELISA, Western Blot and is an excellent antigen for detection of HCV with minimal specificity problems. Other applications not tested.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ug
Source Antigen：Recombinant, E. coli
Grade：Highly Purified
Purity：~95% (10% PAGE, coomassie staining)
Form：Supplied as a liquid in 20mM Tris-Hcl, pH 8.0, 8M urea, 10mM B-ME. No preservative added. Labeled with Biotin.
