85-2065-55　［取扱停止］Heat Shock Protein 65, Recombinant, Mycobacterium Tuberculosis, His-Tag (HSP65, GroEL-2, Cpn60-2, Protein Cpn60-2, groEL protein-2, 65kD Antigen, Cell wall Protein A, Antigen A, GroL2) 10ug　208941

特徴

• Tuberculosis, MTB, or TB (short for tubercle bacillus) is a common, and in many cases lethal, infectious disease caused by various strains of mycobacteria, usually Mycobacterium tuberculosis. The infectious agents of tuberculosis are acid-resistant rod-like bacteria of the family Mycobacteriaceae, genus Mycobacterium. Tuberculosis typically attacks the lungs, but can also affect other parts of the body. It is spread through the air when people who have an active TB infection cough, sneeze, or otherwise transmit their saliva through the air. Individuals with HIV are at risk for infection by tuberculosis due to their impaired immune system.The only currently available vaccine as of 2012 is bacillus Calmette-Guérin (BCG with live attenuated bacteria) which, while it is effective against disseminated disease in childhood, confers inconsistent protection against contracting pulmonary TB. Nevertheless, it is the most widely used vaccine worldwide, with more than 90% of all children being vaccinated. A number of new TB vaccines are currently in phase I and II clinical trials. MVA85A (modified vaccinia Ankara 85A, Oxford University) is a subunit vaccine to BCG. This vaccine produces higher levels of long-lasting cellular immunity when used together with the old TB vaccine called BCG. It uses the attenuated MVA as a vaccine delivery platform to present antigen 85A to the immune system. The closely related proteins of the antigen 85 complex, initially identified in Mycobacterium bovis BCG by crossed immunoelectrophoresis, are major secreted products of mycobacteria growing in synthetic media. Three closely related components, termed antigens 85A, 85B, and 85C, have been demonstrated in M. bovis BCG and M. tuberculosis. Although the antigens are genetically distinct, they are highly homologous and cross-react with polyclonal and monoclonal antibodies raised against individual components. The genes encoding antigen 85A, a 32kD protein also referred to as P32, have been cloned from M. bovis BCG and M. tuberculosis, while genes for 85B, a 30-31kD protein variously termed MPB59 or alpha antigen, have been isolated from M. bovis BCG, Mycobacterium kansasii, and Mycobacterium leprae. Sequence analysis revealed 85% identity between the M. bovis BCG 85A and 85B components in the amino acid sequence of the mature secreted proteins. Many mycobacterial antigens have been identified, such as 71, 65, 38, 23, 19, 16, 14 and 12kD proteins. The 38kD protein is an immunodominant lipoprotein antigen isolated as a component of antigen 5 by affinity chromatography, and is specific only for the M. tuberculosis complex. It is the most extensively studied antigen. The 16kD antigen is an immunodominant antigen, frequently called 14kD, related to the family of low molecular weight heat-shock proteins. This antigen contains B-cell epitopes specific for the M. tuberculosis complex. Heat shock proteins induce pro-inflammatory cytokines. Mycobacterial HSPs participate in cytokine expression resulting from infection by M. tuberculosis. Furthermore, HSPs stabilize cellular proteins in response to various sources of stress or injury. DnaK, originally identified for its DNA replication by bacteriophage l in E. coli is the bacterial hsp70 chaperone. This protein is involved in the folding and assembly of newly synthesized polypeptide chains and in preventing the aggregation of stress-denatured proteins. HSPs may enhance cross- processing by cytosolic or vacuolar mechanisms. Some researchers suggest that endocytosed mammalian HSPs enhance processing by vacuolar mechanisms, and in some cases such processing is TAP independent. Recombinant HSP fusion proteins (with antigenic sequences fused to the N or C terminus of the HSP) have been shown to elicit CD8, T cell and Ab responses.
• Source:Recombinant protein corresponding to Mycobacterium Tuberculosis Heat Shock Protein 65, fused to his-tag at N-terminal, expressed in E. coli.
• Molecular Weight:~57.4kD
• Storage and Stability:May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.