Gamma Aminobutyric acid (GABA) is the major known inhibitory neurotransmitter. The rate-limiting step in the synthesis of GABA is the decarboxylation of glutamate by glutamate decarboxylase (GAD; L-glutamate 1-carboxy-lyase, EC 4.1.115). In the CNS GAB is entirely restricted to GABAergic neurons. GAD is also present in the b-cells of the pancreas and autoantibodies to various GAD polypeptides are detected in insulin-dependent diabetes mellitus. Cloning of GAD genes have identified two subtypes: GAD65 (65kD; human 585aa chromosome 10) and GAD67 (67kD; human 594aa, chromosome 2) share approx. 65% amino acid homology. The N-terminus is the most divergent while the C-terminus is highly conserved. Although both GAD isoforms catalyzes the conversion of GABA but interact differently with the co-factor pyridoxal 5'-phosphate suggesting their activities are differentially regulated. GAD67 is cytosolic, while GAD65 is membrane associated. GAD65 is a major autoantigen in diabetes mellitus and stiff-man syndrome, a rare disease of the brain.
Source:Recombinant protein corresponding to full length mouse GAD65, fused to His-tag, expressed in E. coli.
Molecular Weight:~67kD
Applications:Suitable for use in ELISA, Western Blot as control, Immunohistochemistry as negative control. Other applications not tested.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C. Reconstitute with sterile buffer. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：100ul
Source Antigen：Recombinant, E. coli
Grade：Purified
Purity：Purified
Form：Supplied as a liquid in PBS, pH 7.5. Reconstitute with sterile PBS at ~1mg/ml.
