85-2065-30　［取扱停止］Glucagon, Recombinant, Human (GLP1, GLP2, GRPP) 100ug　208914

特徴

• Glucagon is an important hormone involved in carbohydrate metabolism. The hormone is synthesized and secreted from alpha cells of the islets of Langerhans, which are located in the endocrine portion of the pancreas. Glucagon is released when the glucose level in the blood is low (hypoglycemia), causing the liver to convert stored glycogen into glucose and release it into the bloodstream. The action of glucagon is thus opposite to that of insulin, which instructs the body's cells to take in glucose from the blood in times of satiation. Glucagon is beneficial for the culture of some cell types. It has been used in some biochemical regulation studies of glycogenolysis in hepatocytes. It has been also been found to induce DNA replication in primary cultures of adult rat hepatocytes when used in combinations with EGF and Insulin. Glucagon increases the blood glucose concentration by promoting rapid breakdown of liver glycogen, and also acts to relax smooth muscle such as the gastrointestinal tract.
• Source:Recombinant protein corresponding to human Glucagon, a single, non-glycosylated, polypeptide chain containing 29aa, expressed in E. coli.
• Molecular Weight:~3.483kD
• Biological Activity:Determined by comparing, under certain conditions, the hyperglycemic effect it produces with that produced by the international standard or by reference preparation calibrated in IU and is found to be 1IU/mg.
• AA Sequence:The sequence of the first five N-terminal aa was determined and was found to be His-Ser-Gln-Gly-Thr.
• Storage and Stability:Lyophilized powder may be stored at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.