Fractalkine soluble form is chemotactic for t- cells and monocytes, but not for neutrophils. Fractalkine membrane-bound form promotes adhesion of those leukocytes to endothelial cells. Fractalkine regulates leukocyte adhesion and migration processes at the endothelium and binds to CX3CR1. Natural Human Fractalkine is produced as a long protein (373aa) with an extended mucin-like stalk and a chemokine domain on top. The mucin-like stalk permits it to bind to the cell surface. Fractalkine gene is located on human chromosome 16 along with some CC chemokines known as CCL17and CCL22.
Source:Recombinant protein corresponding to a single, non-glycosylated, polypeptide chain containing 76aa from human Fractalkine, expressed in E. coli.
Molecular Weight:~8638D
Biological Activity:Calculated by its ability to chemoattract human T-Lymphocytes using a concentration range of 5-10ng/ml.
Storage and Stability:Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：5ug
Source Antigen：Recombinant, E. Coli
Grade：Highly Purified
Purity：~97% (RP-HPLC, SDS-PAGE)
Form：Supplied as a lyophilized powder from a 0.2um filtered solution in 20mM phosphate, pH 7.4, 50mM sodium chloride. Reconstitute in 100ul ddH2O for ~1mg/ml stock. For long term storage add a carrier protein (0.1% HSA or BSA).
