85-2065-03　Erbb2, Recombinant, Rhesus Monkey, His-Tag (Her2, Neu) 10ug　208885

特徴

• HER2/neu (also known as ErbB-2, ERBB2) is a protein (protein accession # P04626; 1255aa, ~185kD, chromosome 17q21.1) highly expressed in breast cancers. It is a cell membrane surface-bound receptor tyrosine kinase and is normally involved in the signal transduction pathways leading to cell growth and differentiation. The oncogene neu is so-named because it was derived from a neuroglioblastoma cell line in rat. ErbB2 was named for its similarity to ErbB (avian erythroblastosis oncogene B). Excessive ErbB signaling is associated with the development of a wide variety of types of solid tumor. Insufficient ErbB signaling in humans is associated with the development of neurodegenerative diseases, such as multiple sclerosis and Alzheimer's disease.
• ERBB2 over expression confers resistance to taxol-induced apoptosis by inhibiting p34(CDC2) activation. One mechanism is via ERBB2- mediated up regulation of p21(CIP1), or CDKN1A, which inhibits CDC2. Over expression also occurs in other cancer such as ovarian cancer and stomach cancer. ErbB2 cannot bind growth factors due to the lacking of ligand binding domain of its own and autoinhibited constitutively. Clinically, HER2/neu is important as the target of the monoclonal antibody trastuzumab (marketed as Herceptin). Trastuzumab is only effective in breast cancer where the HER2/neu receptor is over expressed. One of the mechanisms of how traztuzumab works after it binds to HER2 is by increasing p27, a protein that halts cell proliferation. Another monoclonal antibody, pertuzumab, which inhibits dimerization of HER2 and HER3 receptors, is in advanced clinical trials.
• Source:Recombinant protein corresponding to aa1-652 from Rhesus monkey ERBB2, fused to His-tag at C-terminus, expressed in HEK cells.
• Molecular Weight:~105kD under reducing conditions
• Endotoxin:<1EU/ug of protein by LAL method
• Applications:Suitable for use in ELISA and Western Blot. Other applications not tested.
• Recommended Dilution:ELISA: for coating at 1-10ug/mlOptimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.