ER2/neu (also known as ErbB-2, ERBB2) is a protein (protein accession # P04626; 1255aa, ~185kD, chromosome 17q21.1) highly expressed in breast cancers. It is a cell membrane surface-bound receptor tyrosine kinase and is normally involved in the signal transduction pathways leading to cell growth and differentiation. The oncogene neu is so-named because it was derived from a neuroglioblastoma cell line in rat. ErbB2 was named for its similarity to ErbB (avian erythroblastosis oncogene B). Excessive ErbB signaling is associated with the development of a wide variety of types of solid tumor. Insufficient ErbB signaling in humans is associated with the development of neurodegenerative diseases, such as multiple sclerosis and Alzheimer's disease.
Rat ErbB2 is an essential component of a neuregulin-receptor complex, although neuregulins do not interact with it alone. GP30 is a potential ligand for rat ErbB2. It binds to the 5'-TCAAATTC-3' sequence in the MTCO2 promoter and activates its transcription. ErbB2 is widely expressed in epithelial cells, and amplification and/or over expression of ErbB2 has been reported associated with malignancy and a poor prognosis in numerous carcinomas, including breast, prostate and ovarian cancers. ErbB2 cannot bind growth factors due to the lacking of ligand binding domain of its own and auto inhibited constitutively. This receptor forms a heterodimer with other ligand-bound EGF receptor family members, therefore stabilizes ligand binding and enhances kinase-mediated activation of downstream molecules. ErbB2 mediates signaling pathways which involve mitogen- activated protein kinase and phosphatidylinositol-3 kinase, this receptor plays a key role in development, cell proliferation and differentiation.
Source:Recombinant protein corresponding to aa4-656 from rat ERBB2, fused to His-tag at C-terminal, expressed in HEK cells.
Molecular Weight:~110kD under reducing conditions
Endotoxin: <1EU/ug (determined by the LAL method)
Applications:Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilution:ELISA: for coating at 1-10ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：10ug
Source Antigen：Recombinant, HEK cells
Grade：Highly Purified
Purity：Highly Purified (~95%)
Form：Supplied as a liquid in PBS, pH 7.4, 5- 8% trehalose.
