Dengue fever is caused by one of four closely related virus serotypes of the genus Flavivirus, family Flaviviridae, patient may have the second dengue fever infected by the different serotype virus after the primary infection. Detection of specific antibodies to dengue viruses has been used for the diagnosis in clinic. Recently, rapid test has become a most convenient and popular method in the clinical diagnosis. However, there is great difficulty for the manufacturers to have the dengue antigens with the good binding ability to colloid gold, production of strong signal/low background and detection of all four serotype dengue infections for their dengue rapid test development and production.
Product Description:This Dengue antigen product produced in E. coli contains 4 separate Dengue antigen subtypes (1 thru 4), each fused to a 6xHis Tag. The immunoreactive peptides span the viral envelope to the non-functional region. This product consists of 25% recombinant antigen from each of the Dengue subtypes. The sensitivity and specificity for these antigens could reach approximately 93% and 96%, respectively, in Dengue IgG and IgM ELISA.
Molecular Weight:~22KD
Applications:Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ug
Source Antigen：Recombinant, E. coli
Grade：Highly Purified
Purity：>95% (12% PAGE; Coomassie staining).
Form：Supplied as a liquid in PBS, 0.09% sodium azide.
