Cytochrome P450 2D6 is a member of a complex family of microsomal enzymes (mono-oxygenases) present in the endoplasmic reticulum membrane, which perform detoxification reaction on xenobiotic compounds. Cytochrome P450 2D6 is the molecular target of autoantibodies against the "liver kidney microsomal antigen 1" (LKM 1) which has been classically defined by immunofluorescence microscopy. The presence of these autoantibodies is considered indicative of Autoimmune Hepatitis Type 2; LKM 1 antibodies have also been detected in patients with hepatitis C viral infection. The International Autoimmune Hepatitis Group therefore has subdivided the AIH type 2 into two subgroups: 2a with HCV and 2b without HCV. AIH 2a patients are often over 40 and predominantly male. The use of a purified recombinant Cytochrome P450 2D6 antigen allows the differentiation of autoimmune hepatitis from drug-induced hepatitis where transient autoantibodies to other P450 family members occur which cannot be differentiated by immunofluorescence techniques.
Source:Recombinant protein corresponding to human Cytochrome P450 2D6, expressed in Sf9 insect cells.
Immunological Functions:Binds IgG-type human auto-antibodiesStandard ELISA test (checker-board analysis of positive/negative sera panels, including international reference serum NIBSC 66/387).
Molecular Weight:~55.8kD
Applications:Suitable for use in ELISA and Western Blot (with monoclonal anti-LKM-1 antibody and autoimmune hepatitis patient sera). Suitable for Biotinylation and Iodination. Other applications not tested.
Recommended Dilution:ELISA: 0.35-0.6ug/ml (depending on the type of ELISA plate and coating buffer). Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：5ug
Source Antigen：Recombinant, sf9 insect cells
Grade：Purified
Purity：~40% (SDS-PAGE)
Form：Supplied as a liquid in 16mM sodium phosphate buffer, pH 7.6, 400mM sodium chloride, 0.08mM Kl, 20% glycerol.
