The three isoenzymes (MM, MB and BB) are found in muscle, cardiac and brain tissues. These recombinant proteins are ideal for calibrating diagnostic instruments and researching neuromuscular diseases. Creatine kinases can be used for indications in many neuromuscular applications. These disorders include cardiac disease, mitochondrial disorders, inflammatory myopathies, myasthenia, polymyositis, McArdle's disease, NMJ disorders, muscular dystrophy, ALS, hypo and hyperthyroid disorders, central core disease, acid maltase deficiency, myoglobinuria, rhabdomyolysis, motor neuron diseases, rheumatic diseases and others that create elevated or reduced levels of creatine kinases.
Source:Recombinant protein corresponding to human CKMMITIII, a glycosylated polypeptide chain having an identical aa sequence compared to the native enzyme, expressed in Pichia Pastoris.
Biological Activity:Measured by the enzymatic activity of creatine phosphokinase procedure No.45-UV, 1IU-1umole creatine phosphate was 500IU/mg at 37°C.
Applications:Suitable for use in ELISA. Other applications not tested.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：10ug
Source Antigen：Recombinant, Pichia Pastoris
Grade：Highly Purified
Purity：~95% (RP-HPLC, SDS-PAGE)
Form：Supplied as a liquid in 20mM Tris, pH 8, 1mM EDTA, 1mM DTT.
