Creatine Kinase MM is a cytoplasmic enzyme involved in energy homeostasis and is an important serum marker for myocardial infarction. The encoded protein reversibly catalyzes the transfer of phosphate between ATP and various phosphogens such as creatine phosphate. It acts as a homodimer in striated muscle as well as in other tissues, and as a heterodimer with a similar brain isozyme in heart. The encoded protein is a member of the ATP:guanido phosphotransferase protein family.
Source:Recombinant protein corresponding to a glycosylated polypeptide chain with an identical aa sequence as the native enzyme, human CKMMITI, without a C-terminal lysine on both chains, expressed in Pichia Pastoris.
Biological Activity:Measured by the enzymatic activity of creatine phosphokinase procedure No.45-UV, 1IU-1umole creatine phosphate was 500IU/mg at 37°C.
Applications:Suitable for use in ELISA. Other applications not tested.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：10ug
Source Antigen：Recombinant, Pichia Pastoris
Grade：Highly Purified
Purity：~95% (RP-HPLC, SDS-PAGE)
Form：Supplied as a liquid in 0.1M Tris-HCl, pH 7.2, 0.075M sodium chloride, 10mM 2-ME, 0.09% sodium azide, 50% glycerol.
